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Nucleic Acid Purification
Nucleic Acid Purification Search Tool
g-TUBE (10)
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g-TUBE (10)
g-TUBE (10)
g-TUBE (10) from Covaris LLC
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Citations:
(73)
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g-TUBE (10)
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Description
10 individually-wrapped g-TUBES for shearing DNA 6kb to 20 kb and one g-TUBE Prep Station Case quantity available;g-TUBE Case (100) 520104
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
g-TUBE (10)
Company
Covaris LLC
Price
Get Pricing
Get Quote for this Product
Catalog Number
520079
Quantity
10
Format
g-TUBE
Type
tubes
Temperature
Ambient
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Covaris LLC
14 Gill St, Unit H
Woburn
,
Massachusetts
01801-1721
United States
Phone
: +1 781-932-3959
Fax: +1 781-932-8705
Email: info@covarisinc.com
Company Profile
Website:
www.covaris.com
Citations (73)
(1)
Uncovering the true features of dystrophin gene rearrangement and improving the molecular diagnosis of Duchenne and Becker muscular dystrophies
iScience
December 15, 2023
Chao Ling, Yi Dai, Chang Geng, Shirang Pan, Weipeng Quan, Qingyun Ding, Xunzhe Yang, Dongchao Shen, Qing Tao, Jingjing Li,
et al.
constructed as follows: 3 μg of genomic DNA per sample was sheared to 1–6 kb fragments by
g-Tube
(Cat. No.
520079
,
Covaris
, Woburn, MA)
centrifugation
(1,5000 × g, 2 min, twice). DNA was purified after fragment size
(2)
Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis
Sci Rep
December 13, 2023
Daniela Medeot, Analía Sannazzaro, María Julia Estrella, Gonzalo Torres Tejerizo, Bruno Contreras-Moreira, Mariano Pistorio, Edgardo Jofré
(
Thermofisher
PN:
Q32854
) for accurate concentration. Samples were sheared using
Covaris
G-tube
(PN:
520079
) to 20 kb following their recommended protocol. DNA samples were visualized using a Bioanalyzer 12,000
(3)
Neotelomeres and Telomere-Spanning Chromosomal Arm Fusions in Cancer Genomes Revealed by Long-Read Sequencing
bioRxiv
December 1, 2023
Kar-Tong Tan, Michael K. Slevin, Mitchell L. Leibowitz, Max Garrity-Janger, Heng Li, Matthew Meyerson
cat no. SQK-LSK109). Briefly, HMW U2OS DNA was fragmented to ~20 Kb using a
Covaris
®
g-TUBE
(cat no.
520079
) followed by SPRI-cleanup (Agencourt® AMPure XP,
Beckman Coulter
, cat no.
A63881
). Fragmented material
(4)
Long read sequencing characterises a novel structural variant, revealing underactive AKR1C1 with overactive AKR1C2 as a possible cause of severe chronic fatigue
Journal of Translational Medicine
November 17, 2023
Julia Oakley, Martin Hill, Adam Giess, Mélanie Tanguy, Greg Elgar
3 (Diagenode, E07010001 and E07010003) and 10 μg were fragmented to 20-25kb with a gTUBE (
Covaris
,
520079
). All samples were depleted of short DNA fragments (less than 10kb long) with the SRE kit (Circulomics,
(5)
Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics
Cell Genomics
November 8, 2023
Benjamin A. Blount, Xinyu Lu, Maureen R.M. Driessen, Dejana Jovicevic, Mateo I. Sanchez, Klaudia Ciurkot, Yu Zhao, Stephanie Lauer, Robert M. McKiernan, Glen-Oliver F. Gowers,
et al.
Kit,
Qiagen
,Cat#
10243
NEBNext Ultra II FS
DNA Library Prep
Kit (NEB,New England Biolabs,Cat#E7805
g-TUBE
(
Covaris
),
Covaris
,Cat#
520079
Ligation
Sequencing
Kit 1D R9.4,Oxford Nanopore Technologies,Cat#SQK-LSK109
NucleoSpin RNA Plus
Kit,
Macherey-Nagel
,Cat#740984 RNA
(6)
Ultra-fast deep-learned CNS tumour classification during surgery
Nature
October 26, 2023
C. Vermeulen, M. Pagès-Gallego, L. Kester, M. E. G. Kranendonk, P. Wesseling, N. Verburg, P. de Witt Hamer, E. J. Kooi, L. Dankmeijer, J. van der Lugt,
et al.
centred around 5 kb) 3,500 ng of input material in 50 μl is first sheared using a G-Tube (
Covaris
SKU:
520079
), centrifuging at 7,200 RPM (6,000g) in a fixed rotor tabletop centrifuge. Subsequently 7.5 μl of the
(7)
Transcriptome and methylome of the supraoptic nucleus provides insights into the age-dependent loss of neuronal plasticity
Frontiers in Aging Neuroscience
August 30, 2023
Derick Thompson, Abiodun E. Odufuwa, Catherine A. Brissette, John A. Watt
DNA-integrity number between 9.5 and 9.7. DNA was then fragmented using a
Covaris
g-TUBE
(
Covaris
; Cat. #
520079
). Libraries were prepared using the Oxford Nanopore
Ligation
Sequencing
Kit (Oxford; Cat. #SQK-LSK109).
(8)
Fibertools: fast and accurate DNA-m6A calling using single-molecule long-read sequencing
bioRxiv
July 12, 2023
Anupama Jha, Stephanie C. Bohaczuk, Yizi Mao, Jane Ranchalis, Benjamin J. Mallory, Alan T. Min, Morgan O. Hamm, Elliott Swanson, Connor Finkbeiner, Tony Li,
et al.
described (Stergachis et al. 2020). Library preparation Samples were sheared in g-TUBEs (
Covaris
, cat#
520079
) for four passes at 3200 RPM for 2-4 minutes in an Eppendorf 5424R centrifuge. Post-shear samples were
(9)
Long-read genome assembly and gene model annotations for the rodent malaria parasite Plasmodium yoelii 17XNL
Journal of Biological Chemistry
July 1, 2023
Mitchell J. Godin, Aswathy Sebastian, Istvan Albert, Scott E. Lindner
of 2 μg gDNA that was sheared with the
Covaris
g-TUBE
to an average fragment length of 10 kb (Cat #
520079
). The library was sequenced on a PacBio Sequel using a SMRT Cell 1M v3 LR at a 10 pM library loading
(10)
HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcription
Nucleic Acids Research
May 22, 2023
Rui Tian, Zheying Huang, Lifang Li, Jingping Yuan, Qinghua Zhang, Lirong Meng, Bin Lang, Yongfeng Hong, Chaoyue Zhong, Xun Tian,
et al.
data. Nanopore
whole-genome sequencing
HeLa genomic DNA (5 μg) was fragmented to 10 kb by
g-TUBE
(
Covaris
,
520079
) according to the manufacturer's protocol. The
sequencing
library was prepared using the SQK-LSK108
(11)
The complete chloroplast genome of Hibiscus syriacus using long-read sequencing: Comparative analysis to examine the evolution of the tribe Hibisceae
Frontiers in Plant Science
February 2, 2023
Hyunjin Koo, Ah-Young Shin, Seongmin Hong, Yong-Min Kim
brief, gDNA was mechanically sheared to an average size of 20 kb using a
Covaris
g-TUBE
device (Part No.
520079
). In total, 5 μg of sheared gDNA was damage-repaired and end-repaired using polishing enzymes. Blunt-end
(12)
ESPRESSO: Robust discovery and quantification of transcript isoforms from error-prone long-read RNA-seq data
Science Advances
January 20, 2023
Yuan Gao, Feng Wang, Robert Wang, Eric Kutschera, Yang Xu, Stephan Xie, Yuanyuan Wang, Kathryn E. Kadash-Edmondson, Lan Lin, Yi Xing
and
ligation
reaction at 50°C for 60 min. The dsDNA sequences were then sheared by
g-TUBE
(
Covaris
, #
520079
) to the desired size (around 8 to 10 kb) by following the vendor’s instructions. 7)
Library construction
(13)
Long-Read Genome Assembly and Gene Model Annotations for the Rodent Malaria Parasite Plasmodium yoelii 17XNL
bioRxiv
January 11, 2023
Mitchell J. Godin, Aswathy Sebastian, Istvan Albert, Scott E. Lindner
of 2 µg gDNA that was sheared with the
Covaris
g-TUBE
to an average fragment length of 10kb (Cat #
520079
). The library was sequenced on a PacBio Sequel using a SMRT Cell 1M v3 LR at a 10 pM library loading
(14)
Two-speed genome evolution drives pathogenicity in fungal pathogens of animals
Proceedings of the National Academy of Sciences
January 10, 2023
Theresa Wacker, Nicolas Helmstetter, Duncan Wilson, Matthew C. Fisher, David J. Studholme, Rhys A. Farrer
libraries were constructed, one with long unfragmented DNA, one with DNA fragmented to 12 kb with a gTube (
520079
,
Covaris
). The two libraries were loaded onto a single PromethION (FLO-PRO002, type R9.4.1) flowcell
(15)
Simultaneous profiling of histone modifications and DNA methylation via nanopore sequencing
Nature Communications
December 24, 2022
Xue Yue, Zhiyuan Xie, Moran Li, Kai Wang, Xiaojing Li, Xiaoqing Zhang, Jian Yan, Yimeng Yin
NB-900-001-01). Purified genomic DNA was sheared to a fragment of ~10 kb using
Covaris
g-TUBE
(
Covaris
,
520079
), and the fragmented DNA was end-repaired and dA-tailed by combining ~1 μg DNA with
NEBNext FFPE DNA Repair Mix
(16)
Pushing the limits of HiFi assemblies reveals centromere diversity between two Arabidopsis thaliana genomes
Nucleic Acids Research
December 1, 2022
Fernando A Rabanal, Maike Gräff, Christa Lanz, Katrin Fritschi, Victor Llaca, Michelle Lang, Pablo Carbonell-Bejerano, Ian Henderson, Detlef Weigel
HiFi library of Col-0, HMW-DNA (120 ng/μl) was sheared twice (back and forth) with a gTUBE (
Covaris
; #
520079
) in an Eppendorf Centrifuge 5424 at 4,800 rpm (soft) for 3 × 1 min. Five μg of sheared DNA were used
(17)
Comparative Genomics Reveals Evolutionary Drivers of Sessile Life and Left-right Shell Asymmetry in Bivalves
Genomics, Proteomics & Bioinformatics
December 1, 2022
Yang Zhang, Fan Mao, Shu Xiao, Haiyan Yu, Zhiming Xiang, Fei Xu, Jun Li, Lili Wang, Yuanyan Xiong, Mengqiu Chen,
et al.
with Phred quality < 5. PacBio
sequencing
Genomic DNA was sheared by a
g-TUBE
device (Catalog No.
520079
,
Covaris
, MA) with 20 kb settings. Sheared DNA was then purified and concentrated with AMPure XP beads
(18)
Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing
Scientific Reports
December 1, 2022
Surendra Neupane, Sylvia I. Bonilla, Andrew M. Manalo, Kirsten S. Pelz-Stelinski
study). wDi gDNA (4–8 µg in 150 µl TE) was sheared down to 10 kb using Covaris g-TUBES (catalog number
520079
,
Covaris Inc
.), using two passes at 7,000 rpm. The resulting size of the fragments was verified on the
(19)
Clostridia isolated from helminth-colonized humans promote the life cycle of Trichuris species
Cell Reports
November 29, 2022
Shushan Sargsian, Ze Chen, Soo Ching Lee, Amicha Robertson, Rafaela Saes Thur, Julia Sproch, Joseph C. Devlin, Mian Zi Tee, Yi Xian Er, Richard Copin,
et al.
to verify large fragment DNA. Once verified, 1.5ug of DNA was sheared using the
Covaris
G-TUBE
(cat#
520079
) and spun for 2min in each direction at 5.4rpm in the Eppendorf 5415 D centrifuge to shear the DNA to
(20)
CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae
Nature Communications
November 22, 2022
Jun Huang, David Rowe, Pratima Subedi, Wei Zhang, Tyler Suelter, Barbara Valent, David E. Cook
(https://www.protocols.io/view/high-quality-dna-from-
fungi
-for-long-read-sequenci-k6qczdw)94.
g-Tube
(
Covaris
, catalog#
520079
) was used for shearing high-molecular-weight DNA into 20 kb fragments followed by
purification
with
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g-TUBE (10) from Covaris LLC
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